Human Carcinoembryonic Antigen CLIA Kit

Description

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Background

​​​​​​​The Human CEA CLIA Kit is designed, developed, and produced for the quantitative measurement of human CEA level in serum samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of CEA
Assay calibrators, controls, or patient serum samples are added directly to a reaction vessel together with streptavidin coated magnetic particles and biotinylated anti- CEA polyclonal antibody. The magnetic particles capture the biotin antibody as well as an immuno complex in the form of “magnetic particles–biotin CEA antibody–CEA–acridinium ester CEA antibody”. Materials bound to the solid beads are held in a magnetic field while unbound materials are washed away. Then, trigger solutions are added to the reaction vessel and light emission is measured with the ECL100 analyzer. The relative light units (RLU) are proportional to the concentration of a CEA in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum CEA concentration.

Selected Literature

​​​​​​​1.Chen Y M, Wang L, Zhong L H, et al. Application of tumor markers in the early diagnosis of ovarian cancer (J). Current Medicine,2012; 18 (20) : 12-3.
2.Chen Jing-jing, Chen Jian-min, Sun Quan. Clinical significance of CEA, CK19 and SCC in diagnosis of lung cancer [J]. Modern immunology, 2017,37 (3) : 22.
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For in-vitro diagnostic use.